dapi dye liquor (Beyotime)
Structured Review

Dapi Dye Liquor, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 21155 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 21155 article reviews
Images
1) Product Images from "Photoelectrons Mediating Angiogenesis and Immunotherapy through Heterojunction Film for Noninvasive Disinfection"
Article Title: Photoelectrons Mediating Angiogenesis and Immunotherapy through Heterojunction Film for Noninvasive Disinfection
Journal: Advanced Science
doi: 10.1002/advs.202000023
Figure Legend Snippet: a) The cell viability of Ti, GO/Ti, GO/NCDs/Ti, GO/Hap/Ti, and GO/NCDs/Hap/Ti after 1 D and 3 D incubation. b) The cell viability of Ti, GO/Ti, GO/NCDs/Ti, GO/Hap/Ti, and GO/NCDs/Hap/Ti after NIR irradiation (0.5 W cm −2 , 15 min). c) The ALP intensities of Ti, GO/Ti, GO/NCDs/Ti, GO/Hap/Ti, and GO/NCDs/Hap/Ti after 14 D incubation. d) The cells fluorescence staining with FITC (green fluorescence marked cytoplasm) and DAPI (green fluorescence marked cell nucleus). e) The Ca 2+ expressions stained by Fluo‐3 AM probe after NIR irradiation. f) The cells adhesion intensities of Ti, GO/Ti, GO/NCDs/Ti, GO/Hap/Ti, and GO/NCDs/Hap/Ti after NIR irradiation. g) The Ca 2+ expressions of Ti, GO/Ti, GO/NCDs/Ti, GO/Hap/Ti, and GO/NCDs/Hap/Ti after NIR irradiation, h) The cells migration rates of Ti and GO/NCDs/Hap/Ti after NIR irradiation of 0, 12, and 24 h. i) The corresponding mechanism of Ca 2+ flow, which can promote the migration and proliferation as well as ALP enhancement for tissue reconstruction. j) The blood hemolysis rates of Ti, GO/Ti, GO/NCDs/Ti, GO/Hap/Ti, and GO/NCDs/Hap/Ti. k–m) The toxicity detection of BUN, CR, and UA of Ti, GO/NCDs/Ti, and GO/NCDs/Hap/Ti after light treatment.
Techniques Used: Incubation, Irradiation, Fluorescence, Staining, Migration
Figure Legend Snippet: a) The schematic diagram of mild phototherapy through the NIR induced Ca 2+ flow and then activated the PLC γ 1/ERK and PI3K/P‐AKT pathway for injured vessel repair and inflammation relieve. b) The photothermal images in vivo after NIR irradiation (0.5 W cm −2 , 15 min). c) The corresponding spread plates of S. aureus after NIR irradiation with (b). d) The blood routine examination of Ti+L, GO/NCDs/Ti+L, and GO/NCDs/Hap/Ti+L at 1 D treatment. e) The H&E staining of infected tissue near the implant at 1 D treatment. f) The WB analysis regarding to the vascular repair pathway at 3 D treatment. g) The immunofluorescent staining of CD31 (green), DAPI (blue), and α ‐SMA (red) at 6 D treatment.
Techniques Used: In Vivo, Irradiation, Staining, Infection
Figure Legend Snippet: Immune cells number calculation in the blood at 1 D, 2 D, and 3 D treatment after NIR irradiation, a) the WBC, b) Gran, c) Lymph, and d) Mon (grey imaginary line indicated Ti+L, blue imaginary line indicated GO/NCDs/Ti+L, red imaginary line indicated GO/NCDs/Hap/Ti‐L). e) Immunofluorescent staining of IL‐6 and TNF‐ α (green), DAPI (blue), and α ‐SMA (red) after irradiation for 3 D. f) The flow cytometry of CD4 and CD8 lymphocyte in the tissue after irradiation for 3 D. g) The CD3 lymphocyte statistics of CD4 + , CD8 + , and CD4 + /CD8 + , CD4 − /CD8 − . h) The WB analysis regarding to the inflammation relieve at 3 D treatment. i) The corresponding mechanism of mild phototherapy mechanism with GO/NCDs/Hap/Ti film through activating PLC γ 1/ERK and PI3K/P‐AKT pathway.
Techniques Used: Irradiation, Staining, Flow Cytometry